Mammalian Galactose Dehydrogenase

Delayed mammalian meat-induced anaphylaxis due to galactose-α-1,3-galactose in 5 European patients.

Galactose-6-phosphate dehydrogenase. Purification and partial characterization.

A new enzyme, galactose-6-phosphate dehydrogenase has been purified about 50-fold from goat liver. The enzyme can be distinguished from the nonspecific hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase by its high substrate specificity and absolute pyridine nucleotide requirement. In contrast to the hexose-6-phosphate dehydrogenase, this enzyme is located exclusively in the...

NAD(H) recycling activity of an engineered bifunctional enzyme galactose dehydrogenase/lactate dehydrogenase

A chimeric bifunctional enzyme composing of galactose dehydrogenase (galDH; from Pseudomonas fluorescens) and lactate dehydrogenase (LDH; from Bacillus stearothermophilus) was successfully constructed. The chimeric galDH/LDH possessed dual characteristics of both galactose dehydrogenase and lactate dehydrogenase activities while exhibiting hexameric rearrangement with a molecular weight of appr...

Mammalian a-Keto Acid Dehydrogenase Complexes

1. The pig heart pyruvate dehydrogenase complex was separated into lipoamide hydrogenase (&I,~ = 5.55 S) and a colorless fraction by the fractionation on a calcium phosphate gel-cellulose column in the presence of 4 M urea. Subsequently, in the presence of 0.3 M potassium iodide, the colorless fraction was fractionated into two additional components, pyruvate dehydrogenase (7.45 S) and partiall...

Tissue distribution of mammalian lactate dehydrogenase isoenzymes.

system of Jervis [7] is used here.) Previous electrophoretic examination of the LDH of most other plants was carried out with extracts from leaves [1,2j. Such a study of potato leaves would not have revealed the existence of isoenzymic forms in this species. We have, therefore, examined extracts from various regions of a number of plants: roots, stem-base, upper stems, leaves and any special ti...